Journal of Applied Physiology AJP: Renal Physiology
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J Appl Physiol 104: 262-268, 2008. First published October 25, 2007; doi:10.1152/japplphysiol.00893.2007
8750-7587/08 $8.00
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INNOVATIVE METHODOLOGY

Acoustic plethysmography measures breathing in unrestrained neonatal mice

J. Andrew Daubenspeck,1,2 Aihua Li,1 and Eugene E. Nattie1

1Physiology Department, Dartmouth Medical School, Lebanon; and 2Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire

Submitted 20 August 2007 ; accepted in final form 24 October 2007

Measurement of breathing volumes in neonatal mice is of growing importance in order to characterize the influence of development and genetic modifications on respiratory control to evaluate hypotheses concerned with human infant deficits that may affect sudden infant death syndrome, for example. Current techniques require undesirable physical constraints or incur possible artifacts specific to very small animals. We have examined the utility of a recently proposed approach using an acoustic resonance procedure that does not require undue physical constraint beyond placement in the acoustic plethysmograph. We show here that this approach can be applied to baby mice 5 days after birth and that it can be accurately calibrated. In addition, this approach should be useful to study unrestrained neonatal mice under conditions where body temperature approaches environmental temperature and barometric plethysmography cannot be used.

small animal plethysmography; Helmholtz resonator


Address for reprint requests and other correspondence: J. A. Daubenspeck, Dept. of Physiology, Borwell Bldg., Rm. 756E, Dartmouth Medical School, Lebanon, NH 03756 (e-mail: andy.daubenspeck{at}dartmouth.edu)






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