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Apparent diffusion time of oxygen from blood to tissue in rat cerebral cortex: implication for tissue oxygen dynamics during brain functions

¹Department of Radiology and Nuclear Medicine, Akita Research Institute for Brain and Blood Vessels, Akita; ²School of Fundamental Science and Technology, Graduate School of Keio University, Yokohama; ³College of Liberal Arts and Sciences and ⁴Department of Medical Engineering and Technology, Kitasato University, Sagamihara; and ⁵Department of System Design Engineering, Keio University, Yokohama, Japan

Submitted 18 December 2006 ; accepted in final form 10 July 2007

To investigate the dynamics of tissue oxygen demand and supply during brain functions, we simultaneously recorded PO₂ and local cerebral blood flow (LCBF) with an oxygen microelectrode and laser Doppler flowmetry, respectively, in rat somatosensory cortex. Electrical hindlimb stimuli were applied for 1, 2, and 5 s to vary the duration of evoked cerebral metabolic rate of oxygen (CMR_O2). The electrical stimulation induced a robust increase in PO₂ (4–9 Torr at peak) after an increase in LCBF (14–26% at peak). A consistent lag of 1.2 s (0.6–2.3 s for individual animals) in the PO₂ relative to LCBF was found, irrespective of stimulus length. It is argued that the lag in PO₂ was predominantly caused by the time required for oxygen to diffuse through tissue. During brain functions, the supply of fresh oxygen further lagged because of the latency of LCBF onset (0.4 s). The results indicate that the tissue oxygen supports excess demand until the arrival of fresh oxygen. However, a large drop in PO₂ was not observed, indicating that the evoked neural activity demands little extra oxygen or that the time course of excess demand is as slow as the increase in supply. Thus the dynamics of PO₂ during brain functions predominantly depend on the time course of LCBF. Possible factors influencing the lag between demand and supply are discussed, including vascular spacing, reactivity of the vessels, and diffusivity of oxygen.

oxygen transport; cerebral blood flow; functional brain imaging; brain tissue oxygen tension