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Short-term increases in intraluminal pressure reverse age-related decrements in endothelium-dependent dilation in soleus muscle feed arteries

¹Department of Biomedical Sciences, University of Missouri, Columbia, Missouri; and ²Department of Health and Kinesiology, Texas A & M University, College Station, Texas

Submitted 17 April 2007 ; accepted in final form 9 July 2007

We tested the hypothesis that short-term increases in intraluminal pressure improve endothelium-dependent dilation and increase endothelial nitric oxide (NO) synthase (eNOS) expression in senescent soleus muscle feed arteries (SFA). SFA isolated from young (4 mo) and old (24 mo) Fischer 344 rats were cannulated and pressurized at 90 (p90) or 130 (p130) cmH₂O for 4 h. At the end of the 4-h protocol, pressure in p130 SFA was lowered to 90 cmH₂O for examination of endothelium-dependent (flow- or ACh-induced) vasodilation. Flow- and ACh-induced dilations were blunted in old p90 SFA relative to young p90 SFA. Pretreatment with increased pressure (p130) improved flow- and ACh-induced dilations in old SFA, such that vasodilator responses were similar to those in young SFA. In the presence of N-nitro-L-arginine (L-NNA) or L-NNA + indomethacin (Indo), flow-induced dilation was inhibited in old p130 SFA, such that the response was not greater than the response in old p90 SFA. In old p130 SFA, ACh-induced dilation was inhibited by L-NNA + Indo (not L-NNA alone). In a separate experiment, SFA were pressurized at 70, 90, 110, or 130 cmH₂O for 4 h, and eNOS mRNA and protein content were assessed. Increased pressure induced eNOS mRNA expression in young (not old) SFA. eNOS protein content was not altered in young or old SFA. These results indicate that short-term increases in intraluminal pressure improve endothelium-dependent dilation in senescent SFA, in part by enhancing NO bioavailability; however, the beneficial effect was not associated with increased eNOS expression.

endothelial dysfunction; endothelial nitric oxide synthase; exercise

This article has been cited by other articles:

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