Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 102: 2346-2351, 2007. First published January 25, 2007; doi:10.1152/japplphysiol.00822.2006
8750-7587/07 $8.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
102/6/2346    most recent
00822.2006v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rullman, E.
Right arrow Articles by Gustafsson, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rullman, E.
Right arrow Articles by Gustafsson, T.

A single bout of exercise activates matrix metalloproteinase in human skeletal muscle

E. Rullman,1,2 H. Rundqvist,2,3 D. Wågsäter,4 H. Fischer,1,2 P. Eriksson,4 C. J. Sundberg,2 E. Jansson,1 and T. Gustafsson1,2

1Department of Laboratory Medicine, Division of Clinical Physiology, and 2Department of Physiology and Pharmacology, 3Department of Cell and Molecular Biology, and 4Department of Medicine, Atherosclerosis Research Unit, King Gustav V Research Institute, Karolinska University Hospital; Karolinska Institutet, Stockholm, Sweden

Submitted 26 July 2006 ; accepted in final form 25 January 2007

The aims of this study were 1) to characterize changes in matrix metalloproteinase (MMP), endostatin, and vascular endothelial growth factor (VEGF)-A expression in skeletal muscle in response to a single bout of exercise in humans; and 2) to determine if any exchange of endostatin and VEGF-A between circulation and the exercising leg is associated with a change in the tissue expression or plasma concentration of these factors. Ten healthy males performed 65 min of cycle exercise, and muscle biopsies were obtained from the vastus lateralis muscle at rest and immediately and 120 min after exercise. In the muscle biopsies, measurements of mRNA expression levels of MMP-2, MMP-9, MMP-14, and tissue inhibitor of metalloproteinase; VEGF and endostatin protein levels; and MMP activities were performed. Femoral arterial and venous concentrations of VEGF-A and endostatin were determined before, during, and 120 min after exercise. A single bout of exercise increased MMP-9 mRNA and activated MMP-9 protein in skeletal muscle. No measurable increase of endostatin was observed in the skeletal muscle or in plasma following exercise. A concurrent increase in skeletal muscle VEGF-A mRNA and protein levels was induced by exercise, with no signs of peripheral uptake from the circulation. However, a decrease in plasma VEGF-A concentration occurred following exercise. Thus 1) a single bout of exercise activated the MMP system without any resulting change in tissue endostatin protein levels, and 2) the increased VEGF-A protein levels are due to changes in the skeletal muscle tissue itself. Other mechanisms are responsible for the observed exercise-induced decrease in VEGF-A in plasma.

vascular endothelial growth factor-A; endostatin; gene expression; matrix metalloproteinase-9


Address for reprint requests and other correspondence: T. Gustafsson, Div. of Clinical Physiology C1-88, Karolinska Univ. Hospital, Huddinge, 141 86 Stockholm, Sweden (e-mail: thomas.gustafsson{at}ki.se)




This article has been cited by other articles:


Home page
 Eur J EndocrinolHome page
H. Rundqvist, E. Rullman, C. J. Sundberg, H. Fischer, K. Eisleitner, M. Stahlberg, P. Sundblad, E. Jansson, and T. Gustafsson
Activation of the erythropoietin receptor in human skeletal muscle
Eur. J. Endocrinol., September 1, 2009; 161(3): 427 - 434.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. Rullman, J. Norrbom, A. Stromberg, D. Wagsater, H. Rundqvist, T. Haas, and T. Gustafsson
Endurance exercise activates matrix metalloproteinases in human skeletal muscle
J Appl Physiol, March 1, 2009; 106(3): 804 - 812.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. E. Sullivan, C. C. Carroll, B. Jemiolo, S. W. Trappe, S. P. Magnusson, S. Dossing, M. Kjaer, and T. A. Trappe
Effect of acute resistance exercise and sex on human patellar tendon structural and regulatory mRNA expression
J Appl Physiol, February 1, 2009; 106(2): 468 - 475.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Hellsten, N. Rufener, J. J. Nielsen, B. Hoier, P. Krustrup, and J. Bangsbo
Passive leg movement enhances interstitial VEGF protein, endothelial cell proliferation, and eNOS mRNA content in human skeletal muscle
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R975 - R982.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
R. A. Dennis, B. Przybyla, C. Gurley, P. M. Kortebein, P. Simpson, D. H. Sullivan, and C. A. Peterson
Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise
Physiol Genomics, February 19, 2008; 32(3): 393 - 400.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. M. Heinemeier, J. L. Olesen, F. Haddad, H. Langberg, M. Kjaer, K. M. Baldwin, and P. Schjerling
Expression of collagen and related growth factors in rat tendon and skeletal muscle in response to specific contraction types
J. Physiol., August 1, 2007; 582(3): 1303 - 1316.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.