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J Appl Physiol 102: 1936-1944, 2007. First published February 8, 2007; doi:10.1152/japplphysiol.00691.2006
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Importance of pH regulation and lactate/H+ transport capacity for work production during supramaximal exercise in humans

Laurent Messonnier,1 Michael Kristensen,2 Carsten Juel,2 and Christian Denis3

1Equipe Modélisation des Activités Sportives, Département Sciences et Technologies des Activités Physiques et Sportives, Université de Savoie, Le Bourget du Lac, France; 2Copenhagen Muscle Research Centre, Institute of Molecular Biology and Physiology, Copenhagen, Denmark; and 3Laboratoire de Physiologie, Unité Physiologie et Physiopathologie de l’Exercice et Handicap, Université Jean Monnet, Saint-Etienne, France

Submitted 20 June 2006 ; accepted in final form 5 February 2007

We examine the influence of the cytosolic and membrane-bound contents of carbonic anhydrase (CA; CAII, CAIII, CAIV, and CAXIV) and the muscle content of proteins involved in lactate and proton transport [monocarboxylate transporter (MCT) 1, MCT4, and Na+/H+ exchanger 1 (NHE1)] on work capacity during supramaximal exercise. Eight healthy, sedentary subjects performed exercises at 120% of the work rate corresponding to maximal oxygen uptake (Wmax) until exhaustion in placebo (Con) and metabolic alkalosis (Alk) conditions. The total (Wtot) and supramaximal work performed (Wsup) was measured. Muscle biopsies were obtained before and immediately after standardized exercises (se) at 120% Wmax in both conditions to determine the content of the targeted proteins, the decrease in muscle pH ({Delta}pHm), and the muscle lactate accumulation ([Lac]m) per joule of Wsup ({Delta}pHm/Wsup-se and {Delta}[Lac]m/Wsup-se, respectively) and the dynamic buffer capacity. In Con, Wsup was negatively correlated with {Delta}pHm/Wsup-se, positively correlated with {Delta}[Lac]m/Wsup-se and MCT1, and tended to be positively correlated with MCT4 and NHE1. CAII + CAIII were correlated positively with {Delta}pHm/Wsup-se and negatively with {Delta}[Lac]m/Wsup-se, while CAIV was positively related to Wtot. The changes in Wsup with Alk were correlated positively with those in dynamic buffer capacity and negatively with Wsup in Con. Performance improvement with Alk was greater in subjects having a low content of proteins involved in pH regulation and lactate/proton transport. These results show the importance of pH regulating mechanisms and lactate/proton transport on work capacity and the role of the CA to delay decrease in pHm and accumulation in [Lac]m during supramaximal exercise in humans.

metabolic alkalosis; monocarboxylate transporter; sodium/proton exchanger; carbonic anhydrase


Address for reprint requests and other correspondence: L. Messonnier, Equipe Modélisation des Activités Sportives, Département STAPS, Université de Savoie, Campus Universitaire, F-73376 Le Bourget du Lac Cedex, France (e-mail: laurent.messonnier{at}univ-savoie.fr)




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