HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 102/4/1696    most recent 01051.2006v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Radak, Z. Articles by Goto, S. Search for Related Content PubMed PubMed Citation Articles by Radak, Z. Articles by Goto, S.

HIGHLIGHTED TOPIC
Free Radical Biology in Skeletal Muscle

8-Oxoguanosine and uracil repair of nuclear and mitochondrial DNA in red and white skeletal muscle of exercise-trained old rats

¹Institute of Sport Science, Faculty of Physical Education and Sport Science, Semmelweis University, Budapest, Hungary; and ²Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Miyama, Japan

Submitted 20 September 2006 ; accepted in final form 20 December 2006

Oxoguanine DNA glycosylase (OGG1) and uracil DNA glycosylase (UDG) are two of the most important repair enzymes that are involved in the base excision repair processes to eliminate oxidative damage from mammalian DNA, which accumulates with aging. Red and white skeletal muscle fibers have very different antioxidant enzyme activities and resistance to oxidative stress. In this paper, we demonstrate that the activity of OGG1 is significantly higher in the red type of skeletal muscle compared with white fibers from old rats. Exercise training resulted in increased OGG1 activity in the nuclei of red fibers and decreased activity in nuclei of white fibers and in the mitochondria of both red and white fibers. The activities of UDG were similar in both red and white muscle fibers. Exercise training appears to increase the activity of UDG in the nuclei and mitochondria. However, exercise training affects the activity of OGG1 in nuclei and mitochondria differently, suggesting different regulation of the enzymes. In contrast, UDG showed similar activities in nuclei and mitochondrial extracts of exercise-trained animals. These data provide evidence for differential regulation of UDG and OGG1 in maintaining fidelity of DNA in oxidatively stressed cells.

oxoguanine DNA glycosylase; uracil DNA glycosylase; muscle fiber types; oxidative stress

This article has been cited by other articles:

				D. M. Huffman, D. R. Moellering, W. E. Grizzle, C. R. Stockard, M. S. Johnson, and T. R. Nagy Effect of exercise and calorie restriction on biomarkers of aging in mice Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1618 - R1627. [Abstract] [Full Text] [PDF]