HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 102/4/1528    most recent 01054.2006v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Wagner, E. M. Articles by Jenkins, J. Search for Related Content PubMed PubMed Citation Articles by Wagner, E. M. Articles by Jenkins, J.

Effects of airway distension on leukocyte recruitment in the mouse tracheal microvasculature

Department of Medicine, Johns Hopkins University, Baltimore, Maryland

Submitted 20 September 2006 ; accepted in final form 2 January 2007

We have shown previously that excessive distention of the rat trachea during mechanical ventilation results in enhanced leukocyte recruitment to the airway (Lim LH and Wagner EM. Am J Respir Crit Care Med 168:1068–1074, 2003). The objectives of this study were to develop a mouse model of positive end-expiratory pressure (PEEP)-induced leukocyte recruitment to the airway and begin to pursue molecular mechanisms that may contribute to the in vivo observation of increased leukocyte adhesion after PEEP exposure. We studied C57BL/6 wild-type mice and mice deficient in P-selectin or intercellular adhesion molecule-1 (ICAM-1) exposed to intermittent PEEP (8 cmH₂O) applied five times for a 1-min duration, at 10-min intervals. After the imposed ventilatory stress, during normal ventilation (0.2 ml/breath, no PEEP), leukocyte adhesion in tracheal postcapillary venules was determined using intravital microscopy. PEEP induced a time-dependent increase in leukocyte adhesion that was significantly increased between 0 and 60 min (P < 0.01). Furthermore, PEEP-induced leukocyte adhesion at 60 min was ablated in P-selectin- and ICAM-1-deficient mice. These findings demonstrate the essential nature of both P-selectin and ICAM-1 within airway postcapillary venular endothelium for leukocyte recruitment after airway distension.

endothelial activation; intravital microscopy; mechanical stress; positive end-expiratory pressure

This article has been cited by other articles:

				J. Hara, M. Fujimura, A. Ueda, S. Myou, Y. Oribe, N. Ohkura, T. Kita, M. Yasui, and K. Kasahara Effect of Pressure Stress Applied to the Airway on Cough-Reflex Sensitivity in Guinea Pigs Am. J. Respir. Crit. Care Med., March 15, 2008; 177(6): 585 - 592. [Abstract] [Full Text] [PDF]