HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 102/4/1387    most recent 01305.2006v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Moran, A. L. Articles by Lowe, D. A. Search for Related Content PubMed PubMed Citation Articles by Moran, A. L. Articles by Lowe, D. A.

Estradiol replacement reverses ovariectomy-induced muscle contractile and myosin dysfunction in mature female mice

¹School of Kinesiology and ²Department of Physical Medicine and Rehabilitation, University of Minnesota, Minneapolis, Minnesota; and ³Division of Physical Therapy, Georgia State University, Atlanta, Georgia

Submitted 17 November 2006 ; accepted in final form 3 January 2007

Skeletal muscle contractility and myosin function decline following ovariectomy in mature female mice. In the present study we tested the hypothesis that estradiol replacement can reverse those declines. Four-month-old female C57BL/6 mice (n = 69) were ovariectomized (OVX) or sham operated. Some mice were treated immediately with placebo or 17-estradiol (OVX + E₂) while other mice were treated 30 days postsurgery. Thirty or sixty days postsurgery, soleus muscles were assessed in vitro for contractile function and susceptibility to eccentric contraction-induced injury. Myosin structural dynamics was analyzed in extensor digitorum longus (EDL) muscles by electron paramagnetic resonance spectroscopy. Maximal isometric tetanic force was affected by estradiol status (P < 0.001) being 10% less in soleus muscles from OVX compared with sham-operated mice [168 mN (SD 16.7) vs. 180 mN (SD 14.4)] and was restored in OVX + E₂ mice [187 mN (SD 17.6)]. The fraction of strong-binding myosin during contraction was also affected (P = 0.045) and was 15% lower in EDL muscles from OVX compared with OVX + E₂ mice [0.263 (SD 0.034) vs. 0.311 (SD 0.022)]. Plasma estradiol levels were correlated with maximal isometric tetanic force (r = 0.458; P < 0.001) and active stiffness (r = 0.329; P = 0.044), indicating that circulating estradiol influenced muscle and myosin function. Estradiol was not effective in protecting muscle against an acute eccentric contraction-induced injury (P 0.401) but did restore ovariectomy-induced increases in muscle wet mass caused by fluid accumulation. Collectively, estradiol had a beneficial effect on female mouse skeletal muscle.

estrogen; skeletal muscle; muscle force; menopause; hormones

This article has been cited by other articles:

				S. M. Greising, K. A. Baltgalvis, D. A. Lowe, and G. L. Warren Hormone Therapy and Skeletal Muscle Strength: A Meta-Analysis J Gerontol A Biol Sci Med Sci, October 1, 2009; 64A(10): 1071 - 1081. [Abstract] [Full Text] [PDF]

				M. Komrakova, C. Werner, M. Wicke, B. T. Nguyen, S. Sehmisch, M. Tezval, K. M. Stuermer, and E. K. Stuermer Effect of daidzein, 4-methylbenzylidene camphor or estrogen on gastrocnemius muscle of osteoporotic rats undergoing tibia healing period J. Endocrinol., May 1, 2009; 201(2): 253 - 262. [Abstract] [Full Text] [PDF]

				M. Brown, J. Ning, J. A. Ferreira, J. L. Bogener, and D. B. Lubahn Estrogen receptor-{alpha} and -{beta} and aromatase knockout effects on lower limb muscle mass and contractile function in female mice Am J Physiol Endocrinol Metab, April 1, 2009; 296(4): E854 - E861. [Abstract] [Full Text] [PDF]

				M. J. Hubal and P. M. Clarkson Counterpoint: Estrogen and Sex do not Significantly Influence Post-Exercise Indexes of Muscle Damage, Inflammation, and Repair J Appl Physiol, March 1, 2009; 106(3): 1012 - 1014. [Full Text] [PDF]

				F. X. Pizza, B. C. Clark, R. E. De Meersman, S. M. Phillips, N. Stupka, S. Sipila, V. Kovanen, H. Suominen, G. L. Warren, D. A. Lowe, et al. Comments on Point:Counterpoint: Estrogen and sex do/do not influence post-exercise indexes of muscle damage, inflammation, and repair J Appl Physiol, March 1, 2009; 106(3): 1016 - 1020. [Full Text] [PDF]

				G. L. Warren, A. L. Moran, H. A. Hogan, A. S. Lin, R. E. Guldberg, and D. A. Lowe Voluntary run training but not estradiol deficiency alters the tibial bone-soleus muscle functional relationship in mice Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R2015 - R2026. [Abstract] [Full Text] [PDF]