HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 102/3/1078    most recent 00791.2006v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Chow, L. S. Articles by Nair, K. S. Search for Related Content PubMed PubMed Citation Articles by Chow, L. S. Articles by Nair, K. S.

Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function

Division of Endocrinology, Nutrition and Metabolism, Mayo Clinic College of Medicine, Rochester, Minnesota

Submitted 17 July 2006 ; accepted in final form 9 November 2006

We hypothesized that enhanced skeletal muscle mitochondrial function following aerobic exercise training is related to an increase in mitochondrial transcription factors, DNA abundance [mitochondrial DNA (mtDNA)], and mitochondria-related gene transcript levels, as well as spontaneous physical activity (SPA) levels. We report the effects of daily treadmill training on 12-wk-old FVB mice for 5 days/wk over 8 wk at 80% peak O₂ consumption and studied the training effect on changes in body composition, glucose tolerance, muscle mtDNA muscle, mitochondria-related gene transcripts, in vitro muscle mitochondrial ATP production capacity (MATPC), and SPA levels. Compared with the untrained mice, the trained mice had higher peak O₂ consumption (+18%; P < 0.001), lower percentage of abdominal (–25.4%; P < 0.02) and body fat (–19.5%; P < 0.01), improved glucose tolerance (P < 0.04), and higher muscle mitochondrial enzyme activity (+19.5–43.8%; P < 0.04) and MATPC (+28.9 to +32.4%; P < 0.01). Gene array analysis showed significant differences in mRNAs of mitochondria-related ontology groups between the trained and untrained mice. Training also increased muscle mtDNA (+88.4 to +110%; P < 0.05), peroxisome proliferative-activated receptor- coactivator-1 protein (+99.5%; P < 0.04), and mitochondrial transcription factor A mRNA levels (+21.7%; P < 0.004) levels. SPA levels were higher in trained mice (P = 0.056, two-sided t-test) and significantly correlated with two separate substrate-based measurements of MATPC (P < 0.02). In conclusion, aerobic exercise training enhances muscle mitochondrial transcription factors, mtDNA abundance, mitochondria-related gene transcript levels, and mitochondrial function, and this enhancement in mitochondrial function occurs in association with increased SPA.

mouse; endurance exercise; mitochondria adenosine 5'-triphosphate production; gene array; physical activity

This article has been cited by other articles:

				I. R Lanza and K S. Nair Muscle mitochondrial changes with aging and exercise Am. J. Clinical Nutrition, January 1, 2009; 89(1): 467S - 471S. [Abstract] [Full Text] [PDF]

				J. A. Call, K. A. Voelker, A. V. Wolff, R. P. McMillan, N. P. Evans, M. W. Hulver, R. J. Talmadge, and R. W. Grange Endurance capacity in maturing mdx mice is markedly enhanced by combined voluntary wheel running and green tea extract J Appl Physiol, September 1, 2008; 105(3): 923 - 932. [Abstract] [Full Text] [PDF]

				S. V. Brooks, A. Vasilaki, L. M. Larkin, A. McArdle, and M. J. Jackson Repeated bouts of aerobic exercise lead to reductions in skeletal muscle free radical generation and nuclear factor {kappa}B activation J. Physiol., August 15, 2008; 586(16): 3979 - 3990. [Abstract] [Full Text] [PDF]