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J Appl Physiol 101: 1581-1587, 2006. First published July 13, 2006; doi:10.1152/japplphysiol.00426.2006
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Myosin and actin expression and oxidation in aging muscle

LaDora V. Thompson,1 David Durand,1 Nicole A. Fugere,1 and Deborah A. Ferrington2

Departments of 1Physical Medicine and Rehabilitation and 2Ophthalmology, University of Minnesota, Minneapolis, Minnesota

Submitted 11 April 2006 ; accepted in final form 6 July 2006

While the age-related loss in muscle mass partially explains the decline in strength, other yet undefined mechanisms contribute. This study investigates whether changes in myosin-actin stoichiometry and oxidative modification could help explain the decrement in muscle strength with aging. Protein expression and oxidation were evaluated in myosin and actin isolated from the soleus and semimembranosus muscles from young adult, old, and very old Fischer 344 rats. In the soleus muscle, actin and myosin content did not change with aging. In the semimembranosus, actin content was stable, but myosin exhibited decreased content in muscles from very old rats, resulting in a decrease in the myosin-to-actin ratio. 3-Nitrotyrosine and 4-hydroxy-2-nonenal were used as markers of protein oxidative damage. Although myosin and actin are modified with 3-nitrotyrosine and 4-hydroxy-2-nonenal, the extent of chemical modification does not increase with age. The results suggest that the decline in force production with age is not due to the accumulation of these two specific markers of protein oxidation on the myofibrillar proteins. Additionally, age-dependent changes in myofibrillar stoichiometry do not contribute to the decline in force production in the soleus, but may play a role in the semimembranosus with advanced age.

3-nitrotyrosine; 4-hydroxynonenal; skeletal muscle; proteomics


Address for reprint requests and other correspondence: L. V. Thompson, Univ. of Minnesota, 420 Delaware St., S.E., Minneapolis, MN 55455 (e-mail: thomp067{at}umn.edu)




This article has been cited by other articles:


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 J. Gerontol. A Biol. Sci. Med. Sci.Home page
L. M. Snow, N. A. Fugere, and L. V. Thompson
Advanced Glycation End-Product Accumulation and Associated Protein Modification in Type II Skeletal Muscle With Aging
J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2007; 62(11): 1204 - 1210.
[Abstract] [Full Text] [PDF]


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