HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 101/4/1222    most recent 00409.2006v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Whitham, M. Articles by Bishop, N. C. Search for Related Content PubMed PubMed Citation Articles by Whitham, M. Articles by Bishop, N. C.

Effect of caffeine supplementation on the extracellular heat shock protein 72 response to exercise

¹School of Sport, Health and Exercise Sciences, University of Wales, Bangor; and ²School of Sport and Exercise Sciences, Loughborough University, Loughborough, United Kingdom

Submitted 7 April 2006 ; accepted in final form 16 June 2006

The stimulus for the release of 72-kDa heat shock protein (HSP72) during exercise in humans is currently unclear. Recent evidence in an animal model is suggestive of an involvement of catecholamines. The present study, therefore, investigated the effect of caffeine supplementation, a known stimulator of sympathetic activity, on the extracellular (e)HSP72 response to prolonged exercise. Ten healthy male endurance-trained cyclists were recruited (age: 21 ± 1 yr, maximum O₂ uptake 61.1 ± 1.7 ml·kg^–1·min^–1, mean ± SE). Each subject was randomly assigned to ingest either 6 mg/kg body mass of caffeine (Caff) or placebo (Pla) 60 min before one of two 90-min bouts of cycling at 74 ± 1% maximum O₂ uptake. Trials were performed at least 7 days apart in a counterbalanced design. Venous blood samples were collected by venepuncture at pretreatment, preexercise, postexercise, and 1 h postexercise. Serum caffeine and plasma catecholamines were determined using a spectrophotometric assay and high-performance liquid chromatography, respectively. Plasma HSP72 and cortisol were determined by ELISA. Serum caffeine concentrations were significantly increased throughout Caff, while no increases were detected in Pla. Caffeine supplementation and exercise was associated with a greater eHSP72 response than exercise alone (postexercise Caff 8.6 ± 1.3 ng/ml; Pla 5.9 ± 0.9 ng/ml). This greater eHSP72 response was associated with a greater epinephrine response to exercise in Caff. There was a significant increase in norepinephrine and cortisol, with no intertrial differences. The present data suggest that, in humans, catecholamines may be an important mediator of the exercise-induced increase in eHSP72 concentration.

catecholamines; stress; immune

This article has been cited by other articles:

				M. Whitham, S. J. Laing, A. Jackson, N. Maassen, and N. P. Walsh Effect of exercise with and without a thermal clamp on the plasma heat shock protein 72 response J Appl Physiol, October 1, 2007; 103(4): 1251 - 1256. [Abstract] [Full Text] [PDF]