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J Appl Physiol 101: 785-788, 2006. First published May 25, 2006; doi:10.1152/japplphysiol.00135.2006
8750-7587/06 $8.00
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Physical fitness attenuates leukocyte-endothelial adhesion in response to acute exercise

Paul J. Mills,1 Suzi Hong,1 Laura Redwine,2 Steven M. Carter,2 Albert Chiu,2 Michael G. Ziegler,2 Joel E. Dimsdale,1 and Alan S. Maisel2

Departments of 1Psychiatry and 2Medicine, University of California, San Diego, La Jolla, California

Submitted 2 February 2006 ; accepted in final form 8 May 2006

Studies suggest that physical fitness promotes cardiovascular health, including improved endothelial function and possibly reduced inflammatory responses to stressors. This study examined the effects of fitness on leukocyte-endothelial adhesion in response to an acute exercise challenge. Peripheral blood mononuclear cell (PBMC) adhesion to human umbilical venous endothelial cells (HUVEC) was examined in 18 more-fit and 19 less-fit individuals [mean age 39 yr (SD 11)] before and after a 20-min treadmill exercise at 65–70% peak oxygen consumption. PBMC were isolated from whole blood (Ficoll-Paque) at rest and immediately after exercise. HUVEC were incubated for 4 h in the presence of cytokines IL-1 and IL-8 to activate endothelial adhesion molecule expression. Fit subjects showed a significant reduction in PBMC-HUVEC adhesion after exercise (P < 0.01) compared with less-fit subjects, who showed no significant change. Regardless of fitness levels, both at rest and in response to exercise, soluble ICAM-1 in the incubation media attenuated PBMC-HUVEC adhesion by ~81% (P < 0.001). The findings indicate that immune cells that demarginate in response to exercise have reduced ability to adhere in individuals who are physically fit, an effect apparently independent of ICAM-1 binding. The findings provide evidence of how physical fitness might protect individuals from inflammatory responses to exercise.

endothelium; adhesion; soluble intercellular adhesion molecule-1; peripheral blood mononuclear cell


Address for reprint requests and other correspondence: P. J. Mills, UCSD Medical Center, 200 West Arbor Dr., San Diego, CA 92103-0804 (e-mail: pmills{at}ucsd.edu)




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[Abstract] [Full Text] [PDF]


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