HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 100/5/1610    most recent 00828.2005v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Lanças, T. Articles by Dolhnikoff, M. Search for Related Content PubMed PubMed Citation Articles by Lanças, T. Articles by Dolhnikoff, M.

Comparison of early and late responses to antigen of sensitized guinea pig parenchymal lung strips

Departments of ¹Pathology and ²Medicine, School of Medicine, University of São Paulo, São Paulo, Brazil

Submitted 12 July 2005 ; accepted in final form 4 January 2006

The peripheral lung parenchyma has been studied as a component of the asthmatic inflammatory response. During induced constriction, tissue resistance increases in different asthma models. Approximately 60% of the asthmatic patients show early and late responses. The late response is characterized by more severe airway obstruction. In the present study, we evaluated lung parenchymal strips mechanics in ovalbumin-sensitized guinea pigs, trying to reproduce both early and late inflammatory responses. Oscillatory mechanics of lung strips were performed in a control group (C), in an early response group (ER), and in two late response groups: 17 h (L1) and 72 h (L2) after the last ovalbumin challenge. Measurements of resistance and elastance were obtained before and after ovalbumin challenge in C and ER groups and before and after acetylcholine challenge in all groups. Using morphometry, we assessed the density of eosinophils and smooth muscle cells, as well as collagen and elastin content in lung strips. The baseline and postagonist values of resistance and elastance were increased in ER, L1, and L2 groups compared with C (P 0.001). The morphometric analysis showed an increase in alveolar eosinophil density in ER and L2 groups compared with C (P < 0.05). There was a significant correlation between eosinophil density in parenchymal strips of C, L1, and L2 groups and values of resistance and elastance postacetylcholine (r = 0.71, P = 0.001 and r = 0.74, P < 0.001, respectively). The results show that the lung parenchyma is involved in the late response, and the constriction response in this phase is related to the eosinophilic inflammation.

distal lung inflammation; parenchymal oscillatory mechanics; asthma

This article has been cited by other articles:

				R. P. Vieira, V. F. de Andrade, A. C. S. Duarte, A. B. G. dos Santos, T. Mauad, M. A. Martins, M. Dolhnikoff, and C. R. F. Carvalho Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L670 - L679. [Abstract] [Full Text] [PDF]

				A. S. Nakashima, C. M. Prado, T. Lancas, V. C. Ruiz, D. I. Kasahara, E. A. Leick-Maldonado, M. Dolhnikoff, M. A. Martins, and I. F. L. C. Tiberio Oral tolerance attenuates changes in in vitro lung tissue mechanics and extracellular matrix remodeling induced by chronic allergic inflammation in guinea pigs J Appl Physiol, June 1, 2008; 104(6): 1778 - 1785. [Abstract] [Full Text] [PDF]

				P. Angeli, C. M. Prado, D. G. Xisto, P. L. Silva, C. P. Passaro, H. D. Nakazato, E. A. Leick-Maldonado, M. A. Martins, P. R. M. Rocco, and I. F. L. C. Tiberio Effects of chronic L-NAME treatment lung tissue mechanics, eosinophilic and extracellular matrix responses induced by chronic pulmonary inflammation Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1197 - L1205. [Abstract] [Full Text] [PDF]

				R. P. Vieira, A. C. S. Duarte, R. C. Claudino, A. Perini, A. B. G. Santos, H. T. Moriya, F. M. Arantes-Costa, M. A. Martins, C. R. F. Carvalho, and M. Dolhnikoff Creatine Supplementation Exacerbates Allergic Lung Inflammation and Airway Remodeling in Mice Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 660 - 667. [Abstract] [Full Text] [PDF]

				R. P. Vieira, R. C. Claudino, A. C. S. Duarte, A. B. G. Santos, A. Perini, H. C. C. Faria Neto, T. Mauad, M. A. Martins, M. Dolhnikoff, and C. R. F. Carvalho Aerobic Exercise Decreases Chronic Allergic Lung Inflammation and Airway Remodeling in Mice Am. J. Respir. Crit. Care Med., November 1, 2007; 176(9): 871 - 877. [Abstract] [Full Text] [PDF]