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Exercise training in normobaric hypoxia in endurance runners. III. Muscular adjustments of selected gene transcripts

¹Department of Anatomy, University of Bern, 3012 Bern, Switzerland; ²Service de Physiologie Clinique et des Explorations Fonctionnelles Respiratoires et de l'Exercice, Département de Physiologie Équipe d'Accueil 3072, Strasbourg, France; and ³Cardiologie Cellulaire et Moléculaire U-446 Institut National de la Santé et de la Recherche Médicale, Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry, France

Submitted 29 March 2005 ; accepted in final form 29 August 2005

We hypothesized that specific muscular transcript level adaptations participate in the improvement of endurance performances following intermittent hypoxia training in endurance-trained subjects. Fifteen male high-level, long-distance runners integrated a modified living low-training high program comprising two weekly controlled training sessions performed at the second ventilatory threshold for 6 wk into their normal training schedule. The athletes were randomly assigned to either a normoxic (Nor) (inspired O₂ fraction = 20.9%, n = 6) or a hypoxic group exercising under normobaric hypoxia (Hyp) (inspired O₂ fraction = 14.5%, n = 9). Oxygen uptake and speed at second ventilatory threshold, maximal oxygen uptake (O_{2 max}), and time to exhaustion (Tlim) at constant load at O_{2 max} velocity in normoxia and muscular levels of selected mRNAs in biopsies were determined before and after training. O_{2 max} (+5%) and Tlim (+35%) increased specifically in the Hyp group. At the molecular level, mRNA concentrations of the hypoxia-inducible factor 1 (+104%), glucose transporter-4 (+32%), phosphofructokinase (+32%), peroxisome proliferator-activated receptor gamma coactivator 1 (+60%), citrate synthase (+28%), cytochrome oxidase 1 (+74%) and 4 (+36%), carbonic anhydrase-3 (+74%), and manganese superoxide dismutase (+44%) were significantly augmented in muscle after exercise training in Hyp only. Significant correlations were noted between muscular mRNA levels of monocarboxylate transporter-1, carbonic anhydrase-3, glucose transporter-4, and Tlim only in the group of athletes who trained in hypoxia (P < 0.05). Accordingly, the addition of short hypoxic stress to the regular endurance training protocol induces transcriptional adaptations in skeletal muscle of athletic subjects. Expressional adaptations involving redox regulation and glucose uptake are being recognized as a potential molecular pathway, resulting in improved endurance performance in hypoxia-trained subjects.

athletes; messenger ribonucleic acid; redox regulation; glucose uptake

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