Journal of Applied Physiology Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (August 4, 2005). doi:10.1152/japplphysiol.00545.2005
This Article
Right arrow Full Text (PDF) Free
Right arrow Supplemental Tables
Right arrow All Versions of this Article:
99/6/2406    most recent
00545.2005v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Choi, S.
Right arrow Articles by Yan, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choi, S.
Right arrow Articles by Yan, Z.
Submitted on May 10, 2005
Accepted on August 1, 2005

Transcriptional profiling in mouse skeletal muscle following a single bout of voluntary running: evidence of increased cell proliferation

Sangdun Choi1, Xuebin Liu2, Ping Li3, Takayuki Akimoto3, Sun Young Lee1, Mei Zhang3, and Zhen Yan3*

1 Division of Biology, 147-75, California Institute of Technology, Pasadena, CA, USA
2 Departments of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA
3 Department of Medicine, Duke University Medical Center, Durham, NC, USA

* To whom correspondence should be addressed. E-mail: zhen.yan{at}duke.edu.

Skeletal muscle undergoes adaptation following repetitive bouts of exercise. We hypothesize that transcriptional reprogramming and cellular remodeling start in the early phase of long-term training and play an important role in skeletal muscle adaptation. The aim of this study was to define the global mRNA expression in mouse plantaris muscle during (run for 3 and 12 hours) and after (3, 6, 12 and 24 hours post-exercise) a single bout of voluntary running and compare with that after long-term training (4 weeks of running). Among 15,832 gene elements surveyed in a high-density cDNA microarray analysis, 900 showed more than 2-fold changes at one or more time points. K-means clustering and cumulative hypergeometric probability distribution analyses revealed a significant enrichment of genes involved in defense, cell cycle, cell adhesion and motility, signal transduction and apoptosis with induced expression patterns sharing similar patterns with that of peroxisome proliferators activator receptor {gamma} co-activator 1{alpha} (Ppargc1a/Pgc-1{alpha}) and vascular endothelial growth factor A (Vegfa). We focused on the finding of a delayed (at 24 hours post-exercise) induction of mRNA expression of cell cycle genes, origin recognition complex 1, cyclin A2 and cell division 2 homolog A (S. pombe) and confirmed increased cell proliferation by in vivo 5-bromo-2-deoxyuridine (BrdU) labeling following voluntary running. X-ray irradiation of the hindlimb significantly diminished exerciseinduced BrdU incorporation. These findings suggest that a single bout of voluntary running activates the transcriptional network and promotes adaptive processes in skeletal muscle including cell proliferation.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. D. Boppart, S. E. Volker, N. Alexander, D. J. Burkin, and S. J. Kaufman
Exercise promotes {alpha}7 integrin gene transcription and protection of skeletal muscle
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2008; 295(5): R1623 - R1630.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Richard-Bulteau, B. Serrurier, B. Crassous, S. Banzet, A. Peinnequin, X. Bigard, and N. Koulmann
Recovery of skeletal muscle mass after extensive injury: positive effects of increased contractile activity
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C467 - C476.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. Kivela, H. Kyrolainen, H. Selanne, P. V. Komi, H. Kainulainen, and V. Vihko
A single bout of exercise with high mechanical loading induces the expression of Cyr61/CCN1 and CTGF/CCN2 in human skeletal muscle
J Appl Physiol, October 1, 2007; 103(4): 1395 - 1401.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. Y. Chan, W.-J. Qian, D. L. Diamond, T. Liu, M. A. Gritsenko, M. E. Monroe, D. G. Camp II, R. D. Smith, and M. G. Katze
Quantitative Analysis of Human Immunodeficiency Virus Type 1-Infected CD4+ Cell Proteome: Dysregulated Cell Cycle Progression and Nuclear Transport Coincide with Robust Virus Production
J. Virol., July 15, 2007; 81(14): 7571 - 7583.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. F. Kramer and L. J. Goodyear
Exercise, MAPK, and NF-{kappa}B signaling in skeletal muscle
J Appl Physiol, July 1, 2007; 103(1): 388 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. Li, T. Akimoto, M. Zhang, R. S. Williams, and Z. Yan
Resident stem cells are not required for exercise-induced fiber-type switching and angiogenesis but are necessary for activity-dependent muscle growth
Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1461 - C1468.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1948 by the American Physiological Society.